SIRT2 antibody (pAb)

for SIRT2 antibody (pAb)Background

SIRT2 is a member of the SIR2 / Sirtuin family of NAD-dependant deacetylase enzymes. It has multiple targets for deacetylation, including tubulin, P53, HOXA10, FOXO3A, P300, FOXO1. SIRT2 suppresses adipogenesis by deacetylating FOXO1, promoting the binding of FOXO1 to PPARγ and subsequent repression of PPARγ mediated transcriptional activation. Similar to SIRT1, SIRT2 also deacetylates H4AcK16 similar to SIRT1, SIRT2 deacetylates histone H4 at lysine 16. SIRT2 is present in the cytoplasm during most of the cell cycle except in the G2/M transition and in mitosis, where it is found in the nucleus and in contact with chromatin and required for exit from mitosis.

for SIRT2 antibody (pAb)Immunogen

This SIRT2 antibody was raised against a peptide within the C-terminal region of human SIRT2 protein.

for SIRT2 antibody (pAb)Application Notes

This SIRT2 antibody has been validated for use in immunofluorescence, immunoprecipitation and Western blotting. See references for more details.

This antibody reacts only with human SIRT2. This antibody recognizes multiple isoforms of SIRT2, including two species of 40 and 45 kDa.

for SIRT2 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for SIRT2 antibody (pAb)References

This antibody has been used in the following references:
IF: Dryden SC et al (2003) Molec Cell Biol 23: 3173–3185.
WB: Dryden SC et al (2003) Molec Cell Biol 23: 3173–3185.

for SIRT2 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for SIRT2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of arrival.