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ATRX antibody (pAb)

Aliases: alpha thalassemia/mental retardation syndrome X-linked protein, X-linked helicase II, ATP-Dependent Helicase ATRX, Mental Retardation, X-Linked 52
商品コード: 61049 Format: 100 µl ¥67,000 Buy Now
商品コード: 61050 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:300 kDa
Reactivity:Human

Applications

Western Blot Validated

for ATRX antibody (pAb)Application Notes


Validated Applications:
WB: 1:1,000 - 1:5,000 dilution

for ATRX antibody (pAb)Immunogen

This ATRX antibody was raised against a peptide within the C-terminus of human ATRX.

for ATRX antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for ATRX antibody (pAb)References

 
ATRX antibody (pAb) tested by Western blot.

Western blot of ATRX antibody.
Nuclear extract of CCF-STGG1 cells (40 µg per lane) probed with ATRX antibody (pAb) at a dilution of 1:2,500.

for ATRX antibody (pAb)Background

ATRX (alpha thalassemia/mental retardation syndrome X-linked protein, X-linked helicase II, XNP) is a transcriptional regulator and a member of the SWI/SNF family of chromatin remodeling proteins. It contains an SNF2 domain and helicase domain, and a PHD finger. ATRX undergoes cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in interphase gene regulation and chromosomal segregation in mitosis. Mutations of the ATRX gene are associated with an X-linked mental retardation syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes.

for ATRX antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for ATRX antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

ATRX antibody (pAb)
 

Data Thumbnails

ATRX antibody (pAb) tested by Western blot.

Western blot of ATRX pAb.
(Click image to enlarge and see details.)