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BLIMP1 / PRDM1 antibody (pAb)

Aliases: PRDI-BF1
商品コード: 61053 Format: 100 µl ¥67,000 Buy Now
商品コード: 61054 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:110 kDa
Reactivity:Human

Applications

Western Blot Validated

for BLIMP1 / PRDM1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for BLIMP1 / PRDM1 antibody (pAb)Immunogen

This BLIMP1 / PRDM1 antibody was raised against a peptide within the N-terminal half of human BLIMP1 / PRDM1.

for BLIMP1 / PRDM1 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for BLIMP1 / PRDM1 antibody (pAb)References

 
BLIMP1 / PRDM1 antibody (pAb) tested by Western blot.

Western blot of BLIMP1 / PRDM1 antibody.
Nuclear extract of Saos-2 cells (20 µg per lane) probed with BLIMP1 / PRDM1 antibody (pAb) at a dilution of 1:1,000.

for BLIMP1 / PRDM1 antibody (pAb)Background

BLIMP1 / PRDM1 (Beta-interferon gene positive regulatory domain I-binding factor, PR domain-containing protein 1 Positive regulatory domain I-binding factor 1) is a DNA sequence-specific transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene, as well as other genes. It is required for maturation of B-lymphocytes into immunoglobin secreting plasma cells through direct repression of transcription factors such as Pax5, Bcl6, and Myc, which function in the lineage commitment and proliferation of B cells. BLIMP1 / PRDM1 contains an N-terminal SET domain and four C-terminal C2H2-type zinc-finger motifs. It is also required for effector T-cell function and for the specification of primordial germ cell lineages.

for BLIMP1 / PRDM1 antibody (pAb)Positive Control

Active Motif's Saos-2 nuclear extract (Catalog No. 36025) can be used as a positive control for BLIMP1 / PRDM1 antibody.

for BLIMP1 / PRDM1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for BLIMP1 / PRDM1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

BLIMP1 / PRDM1 antibody (pAb)
 

Data Thumbnails

BLIMP1 / PRDM1 antibody (pAb) tested by Western blot.

Western blot of BLIMP1 / PRDM1 pAb.
(Click image to enlarge and see details.)