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Histone H3 acetyl Lys56 antibody (mAb)
| Clone: 12.1 |
| Catalog No: 61061 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 61062 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| Not tested. WB-validated version available: 39281. |
2 - 10 µg per ChIP |
for Histone H3 acetyl Lys56 antibody (mAb) (Clone 12.)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H3 Lys56 acetylation occurs normally during S phase, but disappears in G2. This modification persists in presence of DNA damage and also plays a role in nucleosome assembly. Rtt109 was shown to be the major histone acetyltransferase (HAT) for Lys56 acetylation.
for Histone H3 acetyl Lys56 antibody (mAb) (Clone 12.)Immunogen
This histone H3 acetyl Lys56 antibody was raised against a peptide containing acetyl-Lys56 of human histone H3.
for Histone H3 acetyl Lys56 antibody (mAb) (Clone 12.)Application Notes
This Histone H3 acetyl Lys56 antibody (H3K56Ac) has been validated for use in chromatin IP (ChIP; 2 - 10 µg per ChIP).
for Histone H3 acetyl Lys56 antibody (mAb) (Clone 12.)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
for Histone H3 acetyl Lys56 antibody (mAb) (Clone 12.)Positive Control
Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H3 acetyl Lys56 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 acetyl Lys56 antibody (mAb).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H3 acetyl Lys56 antibody (mAb).
Chromatin IP analysis of Histone H3 acetyl Lys56 antibody.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 5µg of Histone H3 acetyl Lys56 mAb or the equivalent amount of mouse IgG as a negative control. RT-qPCR) was performed on ChIP DNA using a primer pair specific for the PABPC1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
Histone H3 acetyl Lys56 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys56 pAb for acetyl Lys56 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: acetyl-Lys56 peptide.
Lane 2: unmodified H3 peptide.
Lane 3: acetyl-Lys4 peptide.
Lane 4: acetyl-Lys9 peptide.
Lane 5: acetyl-Lys14 peptide.
Lane 6: acetyl-Lys18 peptide.
Lane 7: acetyl-Lys23 peptide.
Lane 8: acetyl-Lys27 peptide.
Lane 9: acetyl-Lys36 peptide.
Lane 10: acetyl-Lys64 peptide.
for Histone H3 acetyl Lys56 antibody (mAb) (Clone 12.)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 acetyl Lys56 antibody (mAb) (Clone 12.)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
