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Histone H3K56ac antibody (mAb)

Clone: 12.1
商品コード: 61061 Format: 100 µg ¥67,000 Buy Now
商品コード: 61062 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:17 kDa
Reactivity:Budding Yeast, Fission Yeast, Human

Applications

ChIP Validated Dot Blot Validated

for Histone H3 acetyl Lys56 antibody (mAb) (Clone 12.1)Application Notes


Validated Applications:
ChIP: 2 - 10 µg per ChIP

This antibody has not been validated for use in Western blotting. Use 39281 instead.

Published Applications: see references

for Histone H3 acetyl Lys56 antibody (mAb) (Clone 12.1)Immunogen

This Histone H3 acetyl Lys56 antibody was raised against a peptide containing acetyl-Lys56 of human histone H3.

for Histone H3 acetyl Lys56 antibody (mAb) (Clone 12.1)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Also available as an AbFlex™ engineered recombinant antibody.  For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91127.

for Histone H3 acetyl Lys56 antibody (mAb) (Clone 12.1)References

 
Histone H3K56ac antibody (mAb) tested by ChIP.

Chromatin IP analysis of Histone H3 acetyl Lys56 antibody.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 5 µg of Histone H3 acetyl Lys56 mAb or the equivalent amount of mouse IgG as a negative control. RT-qPCR) was performed on ChIP DNA using a primer pair specific for the PABPC1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H3K56ac antibody (mAb) tested by dot blot analysis.

Histone H3 acetyl Lys56 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys56 pAb for acetyl Lys56 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row.

Lane 1: acetyl-Lys56 peptide. Lane 2: unmodified H3 peptide. Lane 3: acetyl-Lys4 peptide. Lane 4: acetyl-Lys9 peptide. Lane 5: acetyl-Lys14 peptide. Lane 6: acetyl-Lys18 peptide. Lane 7: acetyl-Lys23 peptide. Lane 8: acetyl-Lys27 peptide. Lane 9: acetyl-Lys36 peptide. Lane 10: acetyl-Lys64 peptide.

for Histone H3 acetyl Lys56 antibody (mAb) (Clone 12.1)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H3 Lys56 acetylation occurs normally during S phase, but disappears in G2. This modification persists in presence of DNA damage and also plays a role in nucleosome assembly. Rtt109 was shown to be the major histone acetyltransferase (HAT) for Lys56 acetylation.

for Histone H3 acetyl Lys56 antibody (mAb) (Clone 12.1)Positive Control

Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H3 acetyl Lys56 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K56ac antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H3K56ac antibody (mAb).

for Histone H3 acetyl Lys56 antibody (mAb) (Clone 12.1)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 acetyl Lys56 antibody (mAb) (Clone 12.1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3K56ac antibody (mAb)
 

Data Thumbnails

Histone H3K56ac antibody (mAb) tested by ChIP.

ChIP of Histone H3K56ac mAb.
(Click image to enlarge and see details.)

Histone H3K56ac antibody (mAb) tested by dot blot analysis.

Dot blot of Histone H3K56ac mAb.
(Click image to enlarge and see details.)