Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
抗体

SMC1-α antibody (pAb)

Aliases: SMC1A, SMCB, CDLS2, SB1.8, SMC1L1, DXS423E, SMC1alpha
商品コード: 61067 Format: 100 µl ¥67,000 Buy Now
商品コード: 61068 Format: 10 µl ¥19,000 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:160 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunoprecipitation Validated

for SMC1-a antibody (pAb)Application Notes


Validated Applications:
ChIP-Seq: 4 µg (7 µl) per ChIP
IP: 10 µl per IP
WB: 1:1,000 - 1:5,000 dilution

for SMC1-a antibody (pAb)Immunogen

This SMC-α antibody was raised against a peptide within the C-terminal region of human SMC1-α.

for SMC1-a antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SMC1-a antibody (pAb)References

 
SMC1-α antibody (pAb) tested by ChIP-Seq.

SMC1-α antibody (pAb) tested by ChIP-Seq.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 15 µg of chromatin from mouse bone marrow cell chromatin and 4 µg SMC1-α antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6.9 million sequence tags were mapped to identify SMC1-α binding sites.

SMC1-α antibody (pAb) tested by immunoprecipitation.

SMC1- α antibody (pAb) tested by Immunoprecipitation.
10 µl of SMC1- α antibody was used to immunoprecipitate SMC1- α from 250 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SMC1- α antibody at a dilution of 1:5,000.

SMC1-α antibody (pAb) tested by Western blot.

SMC1-α antibody (pAb) tested by Western blot.
Nuclear extract (20 µg) of HeLa cells probed with SMC1-α antibody at a dilution of 1:5,000.

for SMC1-a antibody (pAb)Background

The Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. SMC1 has a myosin-like ATPase domain that serves as a molecular motor to help organize chromatin and is part of the cohesin complex that facilitates chromosome cohesion during the cell cycle. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. SMC1 is also involved in DNA damage repair. Subsequent to double strand DNA breaks, SMC1 is phosphorylated by the ATM kinase. Phosphorylated SMC1 is crucial to the successful repair of DNA damage. Defects in SMC1 isoform A are the cause of Cornelia de Lange syndrome type 2, an inherited developmental disorder associated with malformations affecting multiple systems.

for SMC1-a antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for SMC1-α antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with SMC1-α antibody (pAb).

for SMC1-a antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SMC1-a antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SMC1-α antibody (pAb)
 

Data Thumbnails

SMC1-α antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of SMC1-α pAb.
(Click image to enlarge and see details.)

SMC1-α antibody (pAb) tested by immunoprecipitation.

SMC1-α antibody (pAb) tested by immunoprecipitation.
(Click image to enlarge and see details.)

SMC1-α antibody (pAb) tested by Western blot.

Western blot of SMC1-α pAb.
(Click image to enlarge and see details.)