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RBPJ antibody (mAb)

Clone: 7A11
Aliases: SUH, csl, AOS3, CBF1, KBF2, RBP-J, RBPJK, IGKJRB, RBPSUH, IGKJRB1
商品コード: 61073 Format: 100 µg ¥67,000 Buy Now
商品コード: 61074 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2b
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:55 kDa
Reactivity:Human

Applications

Western Blot Validated

for RBPJ antibody (mAb) (Clone 7A11)Application Notes


Validated Applications:
WB: 2 - 4 µg/ml dilution

Published Applications: see references

for RBPJ antibody (mAb) (Clone 7A11)Immunogen

This antibody was raised against full-length recombinant human RBPJ protein.

for RBPJ antibody (mAb) (Clone 7A11)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for RBPJ antibody (mAb) (Clone 7A11)References

 
RBPJ antibody (mAb) tested by Western blot.

Western blot of RBPJ antibody.
Wild type (lane 1) or RBPJ KO (lane 2) DG75 cell lysate was probed with RBPJ antibody (top row) or a GAPDH antibody (bottom row) to show equal loading.

for RBPJ antibody (mAb) (Clone 7A11)Background

RBPJ (Recombination Signal Binding Protein For Immunoglobulin Kappa J Region) is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA.

for RBPJ antibody (mAb) (Clone 7A11)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for RBPJ antibody (mAb) (Clone 7A11)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

RBPJ antibody (mAb)
 

Data Thumbnails

RBPJ antibody (mAb) tested by Western blot.

Western blot of RBPJ mAb.
(Click image to enlarge and see details.)