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PCNA antibody (mAb)
| Clone: 16D10 |
| Catalog No: 61079 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 61080 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| Published. See Application Notes below. |
— | Published. See Application Notes below. |
Published. See Application Notes below. |
for PCNA antibody (mAb) (Clone 16D10)Background
PCNA (Proliferating cell nuclear antigen) is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication.
for PCNA antibody (mAb) (Clone 16D10)Immunogen
This PCNA antibody was raised against full length recombinant human PCNA.
for PCNA antibody (mAb) (Clone 16D10)Application Notes
This PCNA antibody has been validated for use in immunofluorescence (IF), immunoprecipitation (IP) and Western blot. See references for more details.
for PCNA antibody (mAb) (Clone 16D10)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with PCNA antibody (mAb).
for PCNA antibody (mAb) (Clone 16D10)References
This antibody has been used in the following references:
IF: Rottach A et al (2008) Hybridoma 27: 91-98.
IP: Rottach A et al (2008) Hybridoma 27: 91-98.
WB: Spada F et al (2007) J Cell Biol 176: 565-571; Rottach A et al (2008) Hybridoma 27: 91-98.
for PCNA antibody (mAb) (Clone 16D10)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for PCNA antibody (mAb) (Clone 16D10)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
