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RNA pol II antibody (mAb)

Clone: 4F8
Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
商品コード: 61081 Format: 100 µg ¥67,000 Buy Now
商品コード: 61082 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:240 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for RNA pol II antibody (mAb) (Clone 4F8)Application Notes


Published Applications:
ChIP
IP
IF
WB
See references for more information. Individual optimization may be required.

for RNA pol II antibody (mAb) (Clone 4F8)Immunogen

This RNA pol II antibody was raised against a synthetic peptide corresponding to human RNA pol II C-terminal heptad repeat domain.

for RNA pol II antibody (mAb) (Clone 4F8)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for RNA pol II antibody (mAb) (Clone 4F8)References

for RNA pol II antibody (mAb) (Clone 4F8)Background

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

for RNA pol II antibody (mAb) (Clone 4F8)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for RNA pol II antibody.

for RNA pol II antibody (mAb) (Clone 4F8)qPCR Primers

Active Motif's Human Positive Control Primer Set ACTB-2 (Cat. No. 71005) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-2 (Cat. No. 71017) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.

for RNA pol II antibody (mAb) (Clone 4F8)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for RNA pol II antibody (mAb) (Clone 4F8)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

RNA pol II antibody (mAb)