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SIP1 antibody (mAb)
| Clone: 6E5 |
| Catalog No: 61095 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 61096 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| 1 - 4 µg/ml | — | Published. See Application Notes below. |
Published. See Application Notes below. |
for SIP1 antibody (mAb) (Clone 6E5)Background
SIP1 (Smad-interacting protein 1, aka ZEB2, Zinc finger E-box-binding homeobox 2) is a sequence-specific DNA binding protein and transcriptional repressor that is involved in normal embryonic development of neural structures and the neural crest. SIP1 belongs to the delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain proteins and contains a SMAD-binding domain, a homeodomain and two clusters of zinc fingers on the N- and C-termini. SIP1 interacts directly with activated forms of SMAD1, SMAD2 and SMAD3, as well as CBX4 and CTBP1. Mutations in the SIP1 gene cause a form of Hirschsprung disease (HSCR).
for SIP1 antibody (mAb) (Clone 6E5)Immunogen
This SIP1 antibody was raised against a recombinant protein corresponding to amino acids 1-360 of human SIP1.
for SIP1 antibody (mAb) (Clone 6E5)Application Notes
This SIP1 antibody has been validated for use in immunofluorescence, immunohistochemistry, immunoprecipitation and Western blotting (1 - 4 µg/ml). See references for details.
for SIP1 antibody (mAb) (Clone 6E5)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
for SIP1 antibody (mAb) (Clone 6E5)Positive Control
Active Motif's Saos-2 nuclear extract (Catalog No. 36025) can be used as a positive control for SIP1 antibody.
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use SIP1 antibody (mAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with SIP1 antibody (mAb).
for SIP1 antibody (mAb) (Clone 6E5)References
IF: Oztas E et al (2010) Exp Mol Path 89: 182-189.
IHC: Oztas E et al (2010) Exp Mol Path 89: 182-189.
WB: Oztas E et al (2010) Exp Mol Path 89: 182-189.
Western Blot:
Nuclear extract of Saos-2 cells (20 µg per lane) probed with the SIP1 antibody (mAb) at a dilution of 1 µg/ml.
for SIP1 antibody (mAb) (Clone 6E5)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for SIP1 antibody (mAb) (Clone 6E5)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
