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KLF5 antibody (pAb)

Aliases: CKLF, IKLF, BTEB2
商品コード: 61099 Format: 100 µl ¥69,000 Buy Now
商品コード: 61100 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:65 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for KLF5 antibody (pAb)Application Notes


Validated Applications:
ChIP: 5 - 10 µl per ChIP
ChIP-Seq: 5 - 10 µl each
ICC/IF: 1:2,000 dilution
WB: 1:500 - 1:5,000 dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.

For optimal results, primary antibody incubations should be performed at 4°C. Individual optimization may be required.

for KLF5 antibody (pAb)Immunogen

This KLF5 antibody was raised against a peptide derived from the N-terminus of mouse KLF5 (Krueppel-like factor 5).

for KLF5 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for KLF5 antibody (pAb)References

 
KLF5 antibody (pAb) tested by ChIP-Seq.

KLF5 antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from an adenocarcinoma cell line and 5 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 24 million sequence tags were mapped to identify KLF5 binding sites. The image shows binding across a region of chromosome 2. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

KLF5 antibody (pAb) tested by immunofluorescence.

KLF5 antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with KLF5 antibody at a 1:2,000 dilution.

KLF5 antibody (pAb) tested by Western blot.

KLF5 antibody (pAb) tested by Western Blot.
Nuclear extract of K-562 cells (20 µg) probed with the KLF5 antibody (pAb) at a dilution of 1:1,000.

for KLF5 antibody (pAb)Background

KLF5 (Kruppel-like factor 5, IKLF, Basic transcription element-binding protein 2, BTEB2) is a zinc-finger transcription factor that plays a role in cell proliferation, differentiation, cell cycle regulation, angiogenesis, and carcinogenesis and is especially important in epithelial cells. KLF5 (along with KLF2 and KLF4) are highly expressed in undifferentiated mouse embryonic stem cells and downregulated during early stages of differentiation. KLF5 and KLF2 can substitute for KLF4 in the reprogramming of differentiated cells into stem cells. KLF5 has a pro-proliferative role in cells and loss of both alleles of KLF5 is embryonic lethal.

for KLF5 antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for KLF5 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with KLF5 antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with KLF5 antibody (pAb).

for KLF5 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for KLF5 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

KLF5 antibody (pAb)
 

Data Thumbnails

KLF5 antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of KLF5 pAb.
(Click image to enlarge and see details.)

KLF5 antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of KLF5 pAb.
(Click image to enlarge and see details.)

KLF5 antibody (pAb) tested by Western blot.

Western blot of KLF5 pAb.
(Click image to enlarge and see details.)