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Histone H4K8ac antibody (pAb)

商品コード: 61103 Format: 100 µg ¥69,000 Buy Now
商品コード: 61104 Format: 10 µg ¥20,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:8 kDa
Reactivity:Human, Mouse, Other (Wide Range)

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

for Histone H4 acetyl Lys8 antibody (pAb)Application Notes


Validated Applications:
ChIP: 5 - 10 µg per ChIP
ChIP-Seq: 5 - 10 µg each
ICC/IF: 1 µg/ml dilution
WB: 0.1 - 1 µg/ml dilution

Published Applications:

WB

for Histone H4 acetyl Lys8 antibody (pAb)Immunogen

This Histone H4 acetyl Lys8 antibody was raised against a peptide containing acetyl Lys8 of human Histone H4.

for Histone H4 acetyl Lys8 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H4 acetyl Lys8 antibody (pAb)References

 
Histone H4K8ac antibody (pAb) tested by ChIP-Seq.

Histone H4K8ac antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from a human medulloblastoma cell line and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 19 million sequence tags were mapped to identify Histone H4K8ac binding sites. The image shows binding across a region of chromosome 12.

Histone H4K8ac antibody (pAb) tested by ChIP.

Histone H4K8ac antibody (pAb) tested by Chromatin IP.
ChIP performed using HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) and 10 µg of Histone H4 acetyl Lys8 antibody (pAb) or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for either the PABPC1 gene or the GAPDH gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H4K8ac antibody (pAb) tested by immunofluorescence.

Histone H4K8ac antibody (pAb) tested by immunofluorescence.
HeLa cells stained with Histone H4K8ac antibody (green) at a 1 ug/ml dilution. Tubulin staining was done with our alpha Tubulin antibody, catalog no.39527 (red) at a 1:1,000 dilution.

Histone H4K8ac antibody (pAb) tested by Western blot.

Histone H4K8ac antibody (pAb) tested by Western Blot.
HeLa nuclear extract (20 µg per lane) probed with the Histone H4 acetyl Lys8 antibody (0.5 µg per ml). Lane 1: no treatment. Lane 2: cells treated with sodium butyrate.

Histone H4K8ac antibody (pAb) tested by dot blot analysis.

Histone H4K8ac antibody (pAb) tested by Dot blot.
Dot blot analysis was used to confirm the specificity of the acetyl Lys8 histone H4 antibody. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 0.1 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row.

Lane 1: acetyl-Lys5 peptide. Lane 2: acetyl-Lys8 peptide. Lane 3: acetyl-Lys12 peptide. Lane 4: acetyl-Lys16 peptide. Lane 5: acetyl-Lys20 peptide.

for Histone H4 acetyl Lys8 antibody (pAb)Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. The chromatin-remodeling complex SWI/SNF is recruited to promoters through the interaction of the bromodomain of the protein Brg-1, belonging to the SWI/SNF complex, and CBP-acetylated histone H4 Lysine 8, leading to a chromatin remodeling.

for Histone H4 acetyl Lys8 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H4K8ac antibody.

for Histone H4 acetyl Lys8 antibody (pAb)qPCR Primers

Active Motif's Human Positive Control Primer Set ACTB-2 (Cat. No. 71005) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-2 (Cat. No. 71017) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4K8ac antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H4K8ac antibody (pAb).

for Histone H4 acetyl Lys8 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H4 acetyl Lys8 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H4K8ac antibody (pAb)
 

Data Thumbnails

Histone H4K8ac antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of Histone H4K8ac pAb.
(Click image to enlarge and see details.)

Histone H4K8ac antibody (pAb) tested by ChIP.

ChIP of Histone H4K8ac pAb.
(Click image to enlarge and see details.)

Histone H4K8ac antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H4K8ac pAb.
(Click image to enlarge and see details.)

Histone H4K8ac antibody (pAb) tested by Western blot.

Western blot of Histone H4K8ac pAb.
(Click image to enlarge and see details.)

Histone H4K8ac antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H4K8ac pAb.
(Click image to enlarge and see details.)