SMARCAL1 / HARP antibody (mAb)

for SMARCAL1 / HARP antibody (mAb) (Clone 4B10)Background

SMARCAL1 / HARP (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, HepA-related protein, DNA-dependent ATPase A) is a member of the SWI / SNF family of ATPases and chromatin remodeling proteins. Unlike other SWI / SNF family members, SMARCAL1 / HARP has not been shown to remodel chromatin, but possesses an ATP-dependent annealing helicase activity. SMARCAL1 reanneals single stranded DNA bubbles in DNA at DNA replication forks that are bound by Replication Protein A (RPA). Defects in SMARCAL1 / HARP are a cause of Schimke immuno-osseous dysplasia (SIOD) SIOD causes spondyloepiphyseal dysplasia (a disorder of bone growth that results in short stature, skeletal abnormalities, and problems with vision and hearing), renal dysfunction and T-cell immunodeficiency.

for SMARCAL1 / HARP antibody (mAb) (Clone 4B10)Immunogen

This SMARCAL1 / HARP antibody was raised against purifed bovine SMARCAL1 / HARP protein.

for SMARCAL1 / HARP antibody (mAb) (Clone 4B10)Application Notes

This SMARCAL1 / HARP antibody has been validated for use in immunoprecipitation (2 - 10 µg per IP) and Western blotting (0.5 - 2 µg/ml). See references for more details.

for SMARCAL1 / HARP antibody (mAb) (Clone 4B10)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for SMARCAL1 / HARP antibody (mAb) (Clone 4B10)References

IP: Mesner LD et al (1993) Biochemistry 32: 7772-7778.
WB: Mesner LD et al (1991) Biochemistry 30: 11490-11494; Mesner LD et al (1993) Biochemistry 32: 7772-7778.

for SMARCAL1 / HARP antibody (mAb) (Clone 4B10)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for SMARCAL1 / HARP antibody (mAb) (Clone 4B10)Guarantee

This product is guaranteed for 6 months from date of arrival.