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ZEB1 antibody (pAb)
| Catalog No: 61119 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 61120 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:1,000 | — |
for ZEB1 antibody (pAb)Background
ZEB1 (Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein Negative regulator of IL2, NIL-2-A, Transcription factor 8, TCF-8) is a POU domain DNA-binding transcriptional regulator that inhibits the expression of E-cadherin and other epithelial genes central to adhesion and epithelial polarity. ZEB1 induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 positively regulates neuronal differentiation and it represses RCOR1 transcription activation during neurogenesis. Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3).
for ZEB1 antibody (pAb)Immunogen
This ZEB1 antibody was raised against a peptide derived from the N-terminal region of human ZEB1.
for ZEB1 antibody (pAb)Application Notes
This ZEB1 antibody has been validated for use in Western blotting (1:500 - 1:1,000).
for ZEB1 antibody (pAb)Buffer
Purified IgG in PBS, 30% glycerol, 0.035% sodium azide.
for ZEB1 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for ZEB1 antibody.
Western blot of ZEB1 antibody.
Nuclear extract of HeLa cells (40 μg) probed with ZEB1 antibody (1:1,000).
for ZEB1 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for ZEB1 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
