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ZEB1 antibody (pAb)

Aliases: BZP, TCF8, AREB6, FECD6, NIL2A, PPCD3, ZFHEP, ZFHX1A, DELTAEF1
商品コード: 61119 Format: 100 µl ¥67,000 Buy Now
商品コード: 61120 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:200 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for ZEB1 antibody (pAb)Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:1,000 dilution

for ZEB1 antibody (pAb)Immunogen

This ZEB1 antibody was raised against a peptide derived from the N-terminal region of human ZEB1.

for ZEB1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for ZEB1 antibody (pAb)References

 
ZEB1 antibody (pAb) tested by immunoprecipitation.

ZEB1 antibody (pAb) tested by Immunoprecipitation.
10 µl of ZEB1 antibody was used to immunoprecipitate ZEB1 from 250 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ZEB1 antibody at a dilution of 1:1,000.

ZEB1 antibody (pAb) tested by Western blot.

ZEB1 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (40 μg) probed with ZEB1 antibody (1:1,000).

for ZEB1 antibody (pAb)Background

ZEB1 (Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein Negative regulator of IL2, NIL-2-A, Transcription factor 8, TCF-8) is a POU domain DNA-binding transcriptional regulator that inhibits the expression of E-cadherin and other epithelial genes central to adhesion and epithelial polarity. ZEB1 induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 positively regulates neuronal differentiation and it represses RCOR1 transcription activation during neurogenesis. Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3).

for ZEB1 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for ZEB1 antibody.

for ZEB1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for ZEB1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

ZEB1 antibody (pAb)
 

Data Thumbnails

ZEB1 antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of ZEB1 pAb.
(Click image to enlarge and see details.)

ZEB1 antibody (pAb) tested by Western blot.

Western blot of ZEB1 pAb.
(Click image to enlarge and see details.)