Goosecoid antibody (pAb)

RRID: AB_2793515
Aliases: GSC, SAMS
Catalog No: 61121 Format: 100 µl ¥86,000 Buy
Catalog No: 61122 Format: 10 µl ¥24,000 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
40 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:500 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Immunogen

This Goosecoid antibody was raised against a peptide in the N-terminal region of human homeobox protein Goosecoid.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

Goosecoid antibody (pAb) tested by Western blot.
Detection of Goosecoid by Western blot analysis. Lane 1: Nuclear extract of HT-29 cells (30 µg). Lane 2: Nuclear extract of SW48 cells (30 µg).
Both were probed with Goosecoid antibody (pAb) at a 1:500 dilution.

Background

Goosecoid (Gsc) is a homeodomain transcription factor important for vertebrate development and organogenesis. First identified in Xenopus, it is expressed in the area of the Spemann organizer, a region within the embryo that control development and differentiation. Goosecoid regulates cell migration from the Spemann organizer and is overexpressed in a majority of human breast tumors. It is also involved in craniofacial and ribcage development.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting