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TCF7L1 / TCF3 antibody (pAb)
| Catalog No: 61125 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 61126 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | — |
for TCF7L1 / TCF3 antibody (pAb)Background
TCF3 / TCF7L1 (T-cell factor 3, TCF7L1) is a member of the TCF/LEF family and a component of the Wnt signaling pathway and a dominant downstream effector in embryonic stem cells (ESCs). TCF3 binds to DNA and serves as both a repressor as well as an activator of transcription. TCF3 brings developmental signals directly to the core regulatory circuitry of ES cells to influence the balance between pluripotency and differentiation. TCF3 transcriptionally represses many genes important for maintaining pluripotency and self-renewal, as well as those involved in lineage commitment and stem cell differentiation. This effect is in part mediated by the corepressors transducin-like enhancer of split 2 (TLE2) and C-terminal Binding Protein (CtBP).
for TCF7L1 / TCF3 antibody (pAb)Immunogen
This TCF7L1 / TCF3 antibody was raised against a peptide from the N-terminus of human TCF7L1 / TCF3.
for TCF7L1 / TCF3 antibody (pAb)Application Notes
This TCF7L1 / TCF3 antibody has been validated for use in Western blotting (1:500 - 1:2,000).
for TCF7L1 / TCF3 antibody (pAb)Buffer
Purified IgG in PBS, 30% glycerol, 0.035% sodium azide.
Western blot of TCF7L1 / TCF3 antibody.
Nuclear extract of PANC-1 cells (20 μg) probed with TCF7L1 / TCF3 antibody (1:500).
for TCF7L1 / TCF3 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for TCF7L1 / TCF3 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
