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TCF7L1 / TCF3 antibody (pAb)

商品コード: 61125 Format: 100 µl ¥69,000 Buy Now
商品コード: 61126 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:80 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for TCF7L1 / TCF3 antibody (pAb)Application Notes


Validated Applications:
ChIP: 10 µl per ChIP
ChIP-Seq: 10 µl each
WB: 1:500 - 1:2,000 dilution

ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.

for TCF7L1 / TCF3 antibody (pAb)Immunogen

This TCF7L1 / TCF3 antibody was raised against a peptide from the N-terminus of human TCF7L1 / TCF3.

for TCF7L1 / TCF3 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for TCF7L1 / TCF3 antibody (pAb)References

 
TCF7L1 / TCF3 antibody (pAb) tested by ChIP-Seq.

TCF7L1 / TCF3 antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from undifferentiated hESC cells and 7 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 26 million sequence tags were mapped to identify TCF7L1 / TCF3 binding sites. The image shows binding across a region of chromosome 17. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

TCF7L1 / TCF3 antibody (pAb) tested by Western blot.

Western blot of TCF7L1 / TCF3 antibody.
Nuclear extract of PANC-1 cells (20 μg) probed with TCF7L1 / TCF3 antibody (1:500).

for TCF7L1 / TCF3 antibody (pAb)Background

TCF3 / TCF7L1 (T-cell factor 3, TCF7L1) is a member of the TCF/LEF family and a component of the Wnt signaling pathway and a dominant downstream effector in embryonic stem cells (ESCs). TCF3 binds to DNA and serves as both a repressor as well as an activator of transcription. TCF3 brings developmental signals directly to the core regulatory circuitry of ES cells to influence the balance between pluripotency and differentiation. TCF3 transcriptionally represses many genes important for maintaining pluripotency and self-renewal, as well as those involved in lineage commitment and stem cell differentiation. This effect is in part mediated by the corepressors transducin-like enhancer of split 2 (TLE2) and C-terminal Binding Protein (CtBP).

for TCF7L1 / TCF3 antibody (pAb)Positive Control

Active Motif's PANC-1 nuclear extract (Catalog No. 36099) can be used as a positive control for TCF7L1 / TCF3 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with TCF7L1 / TCF3 antibody (pAb).

for TCF7L1 / TCF3 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for TCF7L1 / TCF3 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

TCF7L1 / TCF3 antibody (pAb)
 

Data Thumbnails

TCF7L1 / TCF3 antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of TCF7L1 / TCF3 pAb.
(Click image to enlarge and see details.)

TCF7L1 / TCF3 antibody (pAb) tested by Western blot.

Western blot of TCF7L1 / TCF3 pAb.
(Click image to enlarge and see details.)