DDB1 antibody (pAb)

for DDB1 antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:2,000 dilution

for DDB1 antibody (pAb)Immunogen

This DDB1 antibody was raised against a peptide from the C-terminus of human DDB1.

for DDB1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for DDB1 antibody (pAb)References

for DDB1 antibody (pAb)Background

DDB1 (DNA damage-binding protein 1, HBV X-associated protein 1, XAP-1, Xeroderma pigmentosum group E-complementing protein) is a DNA binding protein that associates with DNA following UV damage and functions in excision repair. In addition to its function in DNA repair, DDB1 plays a role in normal mitotic exit by regulating Anaphase Promoting Complex (APC) activity. Depletion of DDB1 in mitotic cells significantly delays mitotic exit. DDB1 facilitates the binding of substrates to the Cul4-Roc1 complex and the ubiquitination of selected proteins, including histones H2A, H3 and H4 localized to the sites of DNA damage. It is also involved in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of P53 in response to radiation-induced DNA damage and during DNA replication. In mouse studies, it was identified that loss of DDB1 function prevents hepatocytes from replicating DNA, inducing compensatory proliferation of normal hepatocytes that leads to development of hepatocellular carcinoma.

for DDB1 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for DDB1 antibody.

for DDB1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for DDB1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.