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SMC3 antibody (pAb)

Aliases: BAM, BMH, HCAP, CDLS3, CSPG6, SMC3L1
商品コード: 61131 Format: 100 µl ¥67,000 Buy Now
商品コード: 61132 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:150 kDa
Reactivity:Human

Applications

Western Blot Validated

for SMC3 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for SMC3 antibody (pAb)Immunogen

This SMC3 antibody was raised against a peptide in the C-terminal region of human SMC3.

for SMC3 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SMC3 antibody (pAb)References

 
SMC3 antibody (pAb) tested by Western blot.

SMC3 antibody (pAb) tested by Western blot.
Detection of SMC3 by Western blot analysis. Lane 1: Nuclear extract of HeLa cells (20 µg). Lane 2: Nuclear extract of Hep G2 cells (20 µg). Lane 3: Nuclear extract of HEK293 cells (20 µg). Lane 4: Nuclear extract of U2OS cells (20 µg).
All were probed with SMC3 antibody (pAb) at a 1:500 dilution.

for SMC3 antibody (pAb)Background

The Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. SMC3 is a component of the cohesin complex that plays an essential role during chromatid segregation. SMC3 is also involved in DNA recombination and repair, mitotic chromosome organization and microtubule-mediated intracellular transport. SMC3 is acetylated during replication, which is essential for cohesion, and is phosphorylated by ATM subsequent to DNA double strand breakage. SMC3 is frequently elevated in human colon carcinoma and overexpression of the protein transforms fibroblasts.

for SMC3 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for SMC3 antibody.

for SMC3 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SMC3 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SMC3 antibody (pAb)
 

Data Thumbnails

SMC3 antibody (pAb) tested by Western blot.

Western blot of SMC3 pAb.
(Click image to enlarge and see details.)