Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
抗体

Mili / PiwiL2 antibody (mAb)

Clone: 17.8
Aliases: Piwil1l
商品コード: 61143 Format: 100 µg ¥67,000 Buy Now
商品コード: 61144 Format: 10 µg ¥19,000 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:97 kDa
Reactivity:Mouse

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

for Mili / PiwiL2 antibody (mAb) (Clone 17.8)Application Notes


Published Applications:
IP
ICC/IF
WB
See references for more information. Individual optimization may be required.

for Mili / PiwiL2 antibody (mAb) (Clone 17.8)Immunogen

This Mili / PiwiL2 antibody was raised against full-length recombinant mouse MILI / PIWIL2 protein.

for Mili / PiwiL2 antibody (mAb) (Clone 17.8)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Mili / PiwiL2 antibody (mAb) (Clone 17.8)References

for Mili / PiwiL2 antibody (mAb) (Clone 17.8)Background

Mili / PiwiL2 is a member of the PIWI/AGO subfamily of RNA-binding proteins. Mili / PiwiL2 contains a Piwi and an AGO domain and binds a class of RNA called piwi-interacting RNA (piRNA), which are 24 to 30 nucleotides in length and are derived primarily derived from transposons and other repeated sequence elements. Mili / PiwiL2 is required for methylation and inactivation of retrotransposons. Mice deficient in Mili / PiwiL2 are sterile, as it is also required for spermatogenesis. It also positively regulates translation, and is required for germ line stem cell division and differentiation.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Mili / PiwiL2 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Mili / PiwiL2 antibody (mAb).

for Mili / PiwiL2 antibody (mAb) (Clone 17.8)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Mili / PiwiL2 antibody (mAb) (Clone 17.8)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Mili / PiwiL2 antibody (mAb)