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PIM2 antibody (mAb)
| Clone: CHU61 |
| Catalog No: 61159 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 61160 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunoprecipitation |
|---|---|---|
| 0.5 - 2 µg/ml | — | 1 - 10 µg per IP |
for PIM2 antibody (mAb) (Clone CHU61)Background
PIM2 is a serine/threonine protein kinase in the CAMK family. PIM2 promotes cell survival in response to a variety of proliferative signals through activation of the I-κB kinase/NF-κB pathway. It also inhibits apoptosis through the phosphorylation of BAD. PIM2 has been implicated in the transformation of both T and B lymphocytes and is highly expressed in human leukemia and lymphomas, as well as adenocarcinomas and hepatocellular carcinomas.
for PIM2 antibody (mAb) (Clone CHU61)Immunogen
This PIM2 antibody was raised against a recombinant protein corresponding to human PIM2.
for PIM2 antibody (mAb) (Clone CHU61)Application Notes
This PIM2 antibody has been validated for use in immunohistochemistry (paraffin sections only, not frozen sections), immunoprecipitation and Western blotting.
This antibody recognizes three PIM2 isoforms: 34 kDa, 37 kDa and 40 kDa.
for PIM2 antibody (mAb) (Clone CHU61)Buffer
Purified rat IgG in PBS with 30% glycerol and 0.035% sodium azide.
Western blot of PIM2 mAb.
Ramos cell extract (100 µg per lane) probed with PIM2 antibody (mAb).
Immunohistochemistry using PIM2 mAb.
PIM2 detected by immunohistochemistry in paraffin-embedded human tonsil cells.
for PIM2 antibody (mAb) (Clone CHU61)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for PIM2 antibody (mAb) (Clone CHU61)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
