PIM2 antibody (mAb)

RRID: AB_2615012
Clone: CHU61
Aliases: Pim-2 Proto-Oncogene, Serine/Threonine Kinase
Catalog No: 61159 Format: 100 µg ¥60,000 Buy
Catalog No: 61160 Format: 10 µg ¥21,000 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2a
Purification:
Protein G Chromatography
Host:
Rat
Molecular Weight:
37 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunohistochemistry Validated

Application Notes

Applications Validated by Active Motif:
IHC: 2 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

The antibody is suitable for IHC on paraffin sections. Frozen sections are not recommended. This antibody is also available as an AbFlex® engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91239.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This PIM2 antibody was raised against a recombinant protein corresponding to human PIM2.

Buffer

Purified rat IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

Immunohistochemistry using PIM2 mAb.
PIM2 detected by immunohistochemistry in paraffin-embedded human tonsil cells.

Western blot of PIM2 antibody (mAb).
Ramos cell extract (100 µg per lane) probed with PIM2 antibody (mAb).

Background

PIM2 is a serine/threonine protein kinase in the CAMK family. PIM2 promotes cell survival in response to a variety of proliferative signals through activation of the I-κB kinase/NF-κB pathway. It also inhibits apoptosis through the phosphorylation of BAD. PIM2 has been implicated in the transformation of both T and B lymphocytes and is highly expressed in human leukemia and lymphomas, as well as adenocarcinomas and hepatocellular carcinomas.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting