Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
抗体

PBX1b antibody (mAb)

Clone: CT 41.1
Aliases: PBX1
商品コード: 61165 Format: 100 µg ¥67,000 Buy Now
商品コード: 61166 Format: 10 µg ¥19,000 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Monoclonal
Isotype:IgG1k
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:40 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated Immunohistochemistry Validated

for PBX1b antibody (mAb) (Clone CT 41.1)Application Notes


Published Applications:
ChIP
IHC
WB
See references for more information. Individual optimization may be required.

for PBX1b antibody (mAb) (Clone CT 41.1)Immunogen

This PBX1b antibody was raised against a recombinant protein corresponding to amino acids 319 - 347 of human PBX1b.

for PBX1b antibody (mAb) (Clone CT 41.1)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for PBX1b antibody (mAb) (Clone CT 41.1)References

for PBX1b antibody (mAb) (Clone CT 41.1)Background

PBX proteins are a family of TALE-class transcription factors that associate with a subset of Hox proteins, enhancing their DNA binding affinities and specificities. PBX proteins also heterodimerize with the Meis/Prep subfamily of to form trimeric complexes with Hox proteins at specific DNA sites. Amino acids 348-430 are absent in isoform PBX1b.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with PBX1b antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use PBX1b antibody (mAb).

for PBX1b antibody (mAb) (Clone CT 41.1)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PBX1b antibody (mAb) (Clone CT 41.1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PBX1b antibody (mAb)