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PBX1b antibody (mAb)
| Clone: CT 41.1 |
| Catalog No: 61165 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 61166 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunoprecipitation |
|---|---|---|
| Published. See Application Notes below. |
Published. See Application Notes below. |
Published. See Application Notes below. |
for PBX1b antibody (mAb) (Clone CT 41.1)Background
PBX proteins are a family of TALE-class transcription factors that associate with a subset of Hox proteins, enhancing their DNA binding affinities and specificities. PBX proteins also heterodimerize with the Meis/Prep subfamily of to form trimeric complexes with Hox proteins at specific DNA sites. Amino acids 348-430 are absent in isoform PBX1b.
for PBX1b antibody (mAb) (Clone CT 41.1)Immunogen
This PBX1b antibody was raised against a recombinant protein corresponding to amino acids 319 - 347 of human PBX1b.
for PBX1b antibody (mAb) (Clone CT 41.1)Application Notes
This PBX1b antibody has been validated for use in chromatin immunoprecipitation (ChIP), immunofluorescence, immunohistochemistry and Western blotting. See references for more details.
for PBX1b antibody (mAb) (Clone CT 41.1)Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with PBX1b antibody (mAb).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use PBX1b antibody (mAb).
for PBX1b antibody (mAb) (Clone CT 41.1)References
This antibody has been used in the following references:
ChIP: Brendolan A et al (2005) Development 132: 3113 - 3126.
IHC: Park JT et al (2008) Cancer Res 68: 8852 - 8860.
WB: Brendolan A et al (2005) Development 132: 3113 - 3126.
for PBX1b antibody (mAb) (Clone CT 41.1)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for PBX1b antibody (mAb) (Clone CT 41.1)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
