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Antibodies

PRDM1 / BLIMP1 antibody (mAb)

Clone: ROS195
Catalog No: 61167 Format: 100 µg $340 Buy Now
Catalog No: 61168 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG1
Purification:Protein G Chromatography
Applications:FACS, IHC, IP, WB
Reactivity:Human
Molecular Weight:77 & 95 kDa

Applications Table

Western Blot Chromatin IP Immunoprecipitation
Published.
See Application Notes below.		 — Published.
See Application Notes below.

for PRDM1 / BLIMP1 antibody (mAb) (Clone ROS195)Background

PRDM1 / BLIMP1 is a transcriptional repressor that binds to specific target sites in DNA and recruits co-repressors. PRDM1 / BLIMP1 is a master regulator for B cell terminal differentiation, is essential for normal development and immunity and a putative tumor suppressor in diffuse large B cell lymphomas (DLBCLs). PRDM1 / BLIMP1 specifies primordial germ cells through the silencing of the default somatic pathway. PRDM1 / BLIMP1 also acts as a master regulator of intestinal maturation.

for PRDM1 / BLIMP1 antibody (mAb) (Clone ROS195)Immunogen

This PRDM1 / BLIMP1 antibody was raised against full-length recombinant human PRDM1 / BLIMP1 protein.

for PRDM1 / BLIMP1 antibody (mAb) (Clone ROS195)Application Notes

This PRDM1 / BLIMP1 antibody has been validated for use in flow cytometry (FACS), immunohistochemistry, immunoprecipitation and Western blotting. See references for more details. This antibody recognizes both isoforms of PRDM1 / BLIMP1.

for PRDM1 / BLIMP1 antibody (mAb) (Clone ROS195)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use PRDM1 / BLIMP1 antibody (mAb).

for PRDM1 / BLIMP1 antibody (mAb) (Clone ROS195)References

This antibody has been used in the following references:
IHC: Garcia JF et al (2006) Haematologica 91: 467 - 474 ; Nie K et al (2008) Am J Pathol 173: 242 - 252; Liu YY et al (2007) Blood 173: 242 - 252; Zhao WL et al (2008) Blood 111: 3867 - 3871.
WB: Garcia JF et al (2006) Haematologica 91: 467 - 474 ; Nie K et al (2008) Am J Pathol 173: 242 - 252; Liu YY et al (2007) Blood 173: 242 - 252; Zhao WL et al (2008) Blood 111: 3867 - 3871.

for PRDM1 / BLIMP1 antibody (mAb) (Clone ROS195)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for PRDM1 / BLIMP1 antibody (mAb) (Clone ROS195)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

PRDM1 / BLIMP1 antibody (mAb)