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Antibodies

WRN antibody (mAb)

Clone: 195C
Catalog No: 61169 Format: 100 µg $340 Buy Now
Catalog No: 61170 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG1
Purification:Protein G Chromatography
Applications:IF, IHC, WB
Reactivity:Human
Molecular Weight:162 kDa

Applications Table

Western Blot Chromatin IP Immunofluorescence
Published.
See Application Notes below.
Published.
See Application Notes below.

for WRN antibody (mAb) (Clone 195C)Background

WRN protein is a RECQ helicase that contains 3′ to 5′ helicase activity and DNA-dependent ATPase activity. WRN takes part in many processes, including DNA replication, recombination, repair, and telomere maintenance. Mutations in the gene encoding WRN cause an autosomal recessive segmental progeria called Werner syndrome (WS). WS patients prematurely develop atrophic skin, thin gray hair, osteoporosis, type II diabetes, cataracts, arteriosclerosis and cancer.

for WRN antibody (mAb) (Clone 195C)Immunogen

This WRN antibody was raised against a recombinant protein corresponding to amino acids 1072 - 1432 of human WRN.

for WRN antibody (mAb) (Clone 195C)Application Notes

This WRN antibody has been validated for use in immunohistochemistry, immunofluorescence and Western blotting. See references for more details.

for WRN antibody (mAb) (Clone 195C)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with WRN antibody (mAb).

for WRN antibody (mAb) (Clone 195C)References

This antibody has been used in the following references:
IHC: Opresko PL et al (2007) Mech Ageing Dev 128: 4423-4436.
WB: Mao J et al (2010) Cancer Res 70: 6548-6555.

 
WRN antibody (mAb) tested by Western blot.

WRN antibody (mAb) tested by Western blot.
Detection of WRN by Western blot. Lane 1: HeLa cell extract. Lane 2: WS cell extract (fibroblast from a patient with Werner Syndrome).

for WRN antibody (mAb) (Clone 195C)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for WRN antibody (mAb) (Clone 195C)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

WRN antibody (mAb)
 

Data Thumbnails

WRN antibody (mAb) tested by Western blot.

Western blot of WRN mAb.
(Click image to enlarge and see details).