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WRN antibody (mAb)
| RRID: AB_2793536 |
| Clone: 195C |
| Aliases: RECQ3, RECQL2, RECQL3 |
| 商品コード: 61169 | Format: 100 µg | ¥66,800 | Buy |
| 商品コード: 61170 | Format: 10 µg | ¥18,800 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
ICC/IF: 0.5 µg/ml dilution
IHC(P): User optimization required
WB: 0.5 - 2 µg/ml dilution
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
IHC
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
Immunogen
This WRN antibody was raised against a recombinant protein corresponding to amino acids 1072 - 1432 of human WRN.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
WRN antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with WRN antibody at a 0.5 µg/ml dilution.
WRN antibody (mAb) tested by Immunohistochemistry.
Paraffin embedded tissue samples: human tonsil, MDA-MB-231 (breast adenocarcinoma cell line), AG11395 WS (fibroblast of a patient with Werner Syndrome immortalized with SV40), HeLa cells, MRC-5 (lung fibroblast cell line), and MCF-7 (breast carcinoma cell line).
WRN antibody (mAb) tested by Western blot.
Detection of WRN by Western blot. Lane 1: HeLa cell extract. Lane 2: WS cell extract (fibroblast from a patient with Werner Syndrome).
Background
WRN protein is a RECQ helicase that contains 3′ to 5′ helicase activity and DNA-dependent ATPase activity. WRN takes part in many processes, including DNA replication, recombination, repair, and telomere maintenance. Mutations in the gene encoding WRN cause an autosomal recessive segmental progeria called Werner syndrome (WS). WS patients prematurely develop atrophic skin, thin gray hair, osteoporosis, type II diabetes, cataracts, arteriosclerosis and cancer.
Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for WRN antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
