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LXR-β antibody (pAb)

Aliases: NR1H2, NER, UNR, LXRB, NER-I, RIP15
商品コード: 61177 Format: 100 µl ¥69,000 Buy Now
商品コード: 61178 Format: 10 µl ¥20,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:55 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for LXR-β antibody (pAb)Application Notes


Validated Applications:
ChIP: 10 µl per ChIP
ChIP-Seq: 10 µl each
WB: 1:500 - 1:1,000 dilution

Published Applications:
ChIP
ChIP-Seq

for LXR-β antibody (pAb)Immunogen

This LXR-β antibody was raised against a peptide in the N-terminal region of human LXR-β.

for LXR-β antibody (pAb)Buffer

Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for LXR-β antibody (pAb)References

 
LXR-β antibody (pAb) tested by ChIP-chip.

LXR-β pAb tested by ChIP-Chip.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chormatin from 2.5 million primary human liver cells. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image is zoomed in to show LXR-β binding at a known LXR binding site in the RARB promoter.

LXR-β antibody (pAb) tested by ChIP.

LXR-β pAb tested by ChIP-qPCR.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 25 µg of human liver chromatin and 10 µl of LXR-β antibody. ChIP DNA was used in qPCR with the negative control primer pairs and primers against the known LXR binding site in the RARB promoter. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

LXR-β antibody (pAb) tested by Western blot.

LXR-β pAb tested by Western blot.
Detection of LXR-β by Western blot analysis. HeLa whole-cell extract (20 µg) was probed with LXR-β pAb at a 1:1000 dilution.

for LXR-β antibody (pAb)Background

LXR-β (Liver X receptor beta, NR1H2, NER, UNR) is an orphan nuclear receptor and a member of the NR1 receptor family. The LXR receptors (including LXR-α and LXR-β ) bind oxysterols and play key roles in maintaining cholesterol homeostasis in macrophages, primarily by regulating multiple components of the reverse cholesterol transport. They also are potent inhibitors of inflammation and are capable of repressing cytokine and chemokine production by Toll-like receptor (TLR)-activated macrophages. LXR-β is more widely expressed than LXR-α, particularly in the brain.

for LXR-β antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for LXR-β antibody.

for LXR-β antibody (pAb)qPCR Primers

Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with LXR-β antibody (pAb).

for LXR-β antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for LXR-β antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

LXR-β antibody (pAb)
 

Data Thumbnails

LXR-β antibody (pAb) tested by ChIP-chip.

ChIP-chip of LXR-β pAb.
(Click image to enlarge and see details.)

LXR-β antibody (pAb) tested by ChIP.

ChIP of LXR-β pAb.
(Click image to enlarge and see details.)

LXR-β antibody (pAb) tested by Western blot.

Western blot of LXR-β pAb.
(Click image to enlarge and see details.)