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3-Methylcytosine (3-mC) antibody (pAb)
| Catalog No: 61179 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 61180 | Format: 10 µl | $70 | Buy Now |
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for 3-Methylcytosine (3-mC) antibody (pAb)Background
Methylation of DNA can occur non-enzymatically at the nitrogen-three of the cytosine base through spontaneous exposure to endogenous S-adenosyl methionine (SAM). The resulting 3-methylcytosine (3-mC) is mutagenic and must be repaired, which occurs in humans through the base excision repair (BER) or dealkylation via human homologues of the E. coli AlkB protein. 3-methylcytosine is present in human cell lines and increased levels of 3-mC impair proliferation.
for 3-Methylcytosine (3-mC) antibody (pAb)Immunogen
This 3-methylcytosine antibody was raised against 3-methylcytidine conjugated to KLH and recognizes 3-methylcytosine .
for 3-Methylcytosine (3-mC) antibody (pAb)Application Notes
This 3-methylcytosine antibody has been validated for use in dot blot.
for 3-Methylcytosine (3-mC) antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for 3-Methylcytosine (3-mC) antibody (pAb)References
This antibody has been used in the following references:
DB: Dango S et al (2011) Mol Cell 44: 373-384.
3-Methylcytosine (3-mC, 3-methylcytidine) antibody tested by dot blot analysis.
BSA conjugated nucleosides (starting at 10ng as indicated) were spotted onto PVDF membrane and blotted with 3-methylcytidine antibody at a dilution of 1:10,000.
Lane 1: 3-methylcytidine.
Lane 2: Cytidine.
Lane 3: 5-methylcytidine.
Lane 4: 5-hydroxymethylcytodine.
for 3-Methylcytosine (3-mC) antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for 3-Methylcytosine (3-mC) antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
