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CENP-B antibody (pAb)

商品コード: 61183 Format: 100 µg ¥67,000 Buy Now
商品コード: 61184 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:67 kDa
Reactivity:Human

Applications

Immunofluorescence Validated Immunocytochemistry Validated

for CENP-B antibody (pAb)Application Notes


Validated Applications:
ICC/IF: 1 - 2 µg/ml dilution

for CENP-B antibody (pAb)Immunogen

This CENP-B antibody was raised against a recombinant protein corresponding to the C-terminal region of human CENP-B.

for CENP-B antibody (pAb)Buffer

Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for CENP-B antibody (pAb)References

 
CENP-B antibody (pAb) tested by immunofluorescence.

CENP-B pAb tested by immunofluorescence.
Staining of HeLa cells with CENP-B pAb at a 1 µg/ml dilution (green) and α-tubulin (red, Catalog No. 39527) at 1 µg/ml.

for CENP-B antibody (pAb)Background

CENP-B (Centromere protein B) is a protein associated with centromeric DNA and crucial to the formation of the special chromatin structure on the α-satellite DNA repeats of the human centromere. CENP-B specifically binds a 17 bp sequence which may function as a cis-element for centromere-specific nucleosome assembly. CENP-B plays a role in the organization of centromere satellite DNA into a higher order structure, directing centromere formation and kinetochore assembly in mammalian chromosomes.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CENP-B antibody (pAb).

for CENP-B antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for CENP-B antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

CENP-B antibody (pAb)
 

Data Thumbnails

CENP-B antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of CENP-B pAb.
(Click image to enlarge and see details.)