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LIN28A antibody (pAb)
| RRID: AB_2793546 |
| Aliases: CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A |
| 商品コード: 61191 | Format: 100 µl | ¥66,800 | Buy |
| 商品コード: 61192 | Format: 10 µl | ¥18,800 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
ICC/IF: 1:200 dilution
WB: 1:500 - 1:1,000 dilution
Immunogen
This LIN28A antibody was raised against a peptide derived from the C-terminal region of human LIN28A.
Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Immunocytochemical analysis of LIN28A staining of mouse embryonic stem cells.
Mouse embryonic stem cells (mESCs) grown on mouse embryonic fibroblast feeder cells (MEFs) were fixed with 4% paraformaldehyde for 10 minutes at room temperature. Cells were then permeabilized and blocked by incubating with Blocking Solution containing 5% serum/0.1% Triton X-100 in D-PBS for 2 hours at room temperature. Cells were then incubated with LIN28A antibody (Catalog No. 61191, red) at 1:200 dilution overnight at 4°C, washed with D-PBS, and incubated for 2 hours at room temperature with goat anti-mouse Cy3 secondary antibody at 1:250 dilution. Nuclei were stained with DAPI (blue). Cells were visualized using a Zeiss fluorescent microscope at 20X magnification. Images show that LIN28A antibody specifically stains mESC colonies and does not stain MEFs. Absence of LIN28A staining in a subset of cells within the colonies suggests differentiation. Scale bars, 100 μm.
LIN28A antibody (pAb) tested by Western blot.
Cytosolic extract (40 µg) of mouse ES cells probed with LIN28A antibody at a dilution of 1:500.
Background
LIN28 is an evolutionarily conserved RNA-binding protein that serves as a marker of undifferentiated human embryonic stem cells. LIN28 can facilitate the reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
