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Antibodies

HSC70 / HSP7C antibody (mAb)

Clone: 6E1
Catalog No: 61207 Format: 100 µg $340 Buy Now
Catalog No: 61208 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Rat
Isotype:IgG2a
Purification:Protein G Chromatography
Applications:IF, IP, WB
Reactivity:Human
Molecular Weight:70 kDa

Applications Table

Western Blot Chromatin IP IF IP
Published.
See Application Notes below.
Published.
See Application Notes below.
Published.
See Application Notes below.

for HSC70 / HSP7C antibody (mAb) (Clone 6E1)Background

HSC70 / HSP7C (Heat Shock Cognate 70, HSP73, Heat Shock Protein 73) is a member of the DnaK/HSP70 family of protein chaperones involved in protein homeostasis. HSC70 is constitutively expressed and essential for cell viability and functions both in preventing or reversing protein aggregation and in disassembling protein complexes. HSC70 predominantly binds non-native polypeptides and folding intermediates that are either newly synthesized or stress-induced.

for HSC70 / HSP7C antibody (mAb) (Clone 6E1)Immunogen

This HSC70 / HSP7C antibody was raised against full-length recombinant human HSC70 / HSP7C protein.

for HSC70 / HSP7C antibody (mAb) (Clone 6E1)Application Notes

This HSC70 / HSP7C antibody has been validated for use in Western blotting, immunoprecipitation and immunofluorescence. See references for more details.

for HSC70 / HSP7C antibody (mAb) (Clone 6E1)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with HSC70 / HSP7C antibody (mAb).

for HSC70 / HSP7C antibody (mAb) (Clone 6E1)References

This use of this antibody is described in the following references:
IF: Shiota M et al (2010) Hybridoma 29: 453-456.
IP: Shiota M et al (2010) Hybridoma 29: 453-456.
WB: Shiota M et al (2010) Hybridoma 29: 453-456.

for HSC70 / HSP7C antibody (mAb) (Clone 6E1)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for HSC70 / HSP7C antibody (mAb) (Clone 6E1)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

HSC70 / HSP7C antibody (mAb)