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Antibodies

HSP72 antibody (mAb)

Clone: 3G7
Catalog No: 61209 Format: 100 µg $340 Buy Now
Catalog No: 61210 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Rat
Isotype:IgG2a
Purification:Protein G Chromatography
Applications:IF, IP, WB
Reactivity:Human
Molecular Weight:70 kDa

Applications Table

Western Blot Chromatin IP IF IP
Published.
See Application Notes below.
Published.
See Application Notes below.
Published.
See Application Notes below.

for HSP72 antibody (mAb) (Clone 3G7)Background

HSP72 (Heat Shock Protein 72) is a member of the DnaK/HSP70 family of protein chaperones involved in protein homeostasis. HSP72 is expressed at low constitutive levels and is induced upon exposure to environmental stress. HSP72 released into the blood can serve as an alert for the immune system. Increased levels of HSP72 can repress expression of inflammatory response genes by inhibiting NF-κB.

for HSP72 antibody (mAb) (Clone 3G7)Immunogen

This HSP72 antibody was raised against full-length recombinant human HSP72 protein.

for HSP72 antibody (mAb) (Clone 3G7)Application Notes

This HSP72 antibody has been validated for use in Western blotting, immunoprecipitation and immunofluorescence. See references for more details.

for HSP72 antibody (mAb) (Clone 3G7)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with HSP72 antibody (mAb).

for HSP72 antibody (mAb) (Clone 3G7)References

This use of this antibody is described in the following references:
IF: Tanaka M et al (2011) Hybridoma 30: 397-400.
IP: Tanaka M et al (2011) Hybridoma 30: 397-400.
WB: Tanaka M et al (2011) Hybridoma 30: 397-400.

for HSP72 antibody (mAb) (Clone 3G7)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for HSP72 antibody (mAb) (Clone 3G7)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting