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GLI1 antibody (pAb)
| Catalog No: 61215 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 61216 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | — |
for GLI1 antibody (pAb)Background
GLI1 (Glioma-associated oncogene 1) is a zinc finger transcription factor and oncogene. GLI1 is part of the hedgehog signaling pathway, but is also activated in response to TGF-β signaling as well. GLI1 activates transcription of a number of genes that promote cell cycle progression. GLI1 is overexpressed as a result of constitutive activation of hedgehog signaling, which plays a role in the initiation of cancer. GLI1 also promotes self-renewal of neural stem cells.
for GLI1 antibody (pAb)Immunogen
This GLI1 antibody was raised against a peptide within the C-terminal region of human GLI1.
for GLI1 antibody (pAb)Application Notes
This GLI1 antibody has been validated for use in Western blotting (1:500 - 1:2,000).
for GLI1 antibody (pAb)Buffer
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.
for GLI1 antibody (pAb)Positive Control
Active Motif's Raji nuclear extract (Catalog No. 36023) can be used as a positive control for GLI1 antibody.
GLI1 antibody (pAb) tested by Western blot.
Nuclear extract of Raji cells (20 µg) probed with GLI1 antibody (pAb) at a dilution of 1:1,000.
for GLI1 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for GLI1 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
