Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
抗体

PHB / Prohibitin antibody (pAb)

Aliases: PHB1, HEL-215, HEL-S-54e
商品コード: 61217 Format: 100 µl ¥67,000 Buy Now
商品コード: 61218 Format: 10 µl ¥19,000 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:30 kDa
Reactivity:Human

Applications

Western Blot Validated

for PHB / Prohibitin antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

For optimal results, primary antibody incubations should be performed at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for PHB / Prohibitin antibody (pAb)Immunogen

This PHB / prohibitin antibody was raised against a peptide within the N-terminal region of human PHB.

for PHB / Prohibitin antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for PHB / Prohibitin antibody (pAb)References

 
PHB / Prohibitin antibody (pAb) tested by Western blot.

PHB / Prohibitin antibody (pAb) tested by Western blot.
Whole-cell extract of MCF-7 cells (25 µg) probed with PHB antibody (pAb) at a dilution of 1:500.

for PHB / Prohibitin antibody (pAb)Background

PHB / Prohibitin is a key cell cycle regulatory protein, inhibiting cell division by inactivating E2F family proteins, and has potent anti-proliferative activity. PHB / Prohibitin is also involved in repression of transcription through androgen receptor.

for PHB / Prohibitin antibody (pAb)Positive Control

Active Motif's MCF-7 nuclear extract (Catalog No. 36017) can be used as a positive control for PHB / Prohibitin antibody.

for PHB / Prohibitin antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PHB / Prohibitin antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PHB / Prohibitin antibody (pAb)
 

Data Thumbnails

PHB / Prohibitin antibody (pAb) tested by Western blot.

Western blot of PHB / Prohibitin pAb.
(Click image to enlarge and see details.)