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JMJD2B / KDM4B antibody (pAb)

Aliases: TDRD14B
商品コード: 61221 Format: 100 µl ¥67,000 Buy Now
商品コード: 61222 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:142 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

for JMJD2B / KDM4B antibody (pAb)Application Notes


Validated Applications:
ICC/IF: 1:2,000 dilution
IP: 10 µl per IP
WB: 1:500 - 1:1,000 dilution

For optimal results, primary antibody incubations should be performed at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for JMJD2B / KDM4B antibody (pAb)Immunogen

This JMJD2B / KDM4B antibody was raised against a peptide within the C-terminal region of human JMJD2B.

for JMJD2B / KDM4B antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for JMJD2B / KDM4B antibody (pAb)References

 
JMJD2B / KDM4B antibody (pAb) tested by immunofluorescence.

JMJD2B / KDM4B antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with JMJD2B / KDM4B antibody at a 1:2,000 dilution.

JMJD2B / KDM4B antibody (pAb) tested by immunoprecipitation.

JMJD2B / KDM4B antibody (pAb) tested by Immunoprecipitation.
10 µl of JMJD2B / KDM4B antibody was used to immunoprecipitate JMJD2B / KDM4B from 500 µg of K562 whole cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the JMJD2B / KDM4B antibody at a dilution of 1:500.

JMJD2B / KDM4B antibody (pAb) tested by Western blot.

JMJD2B / KDM4B antibody (pAb) tested by Western blot.
Nuclear extract of K-562 cells (40 µg) probed with JMJD2B antibody (pAb) at a dilution of 1:500.

for JMJD2B / KDM4B antibody (pAb)Background

JMJD2B / KDM4B (JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, JHDM3B) is a histone demethylase that has been shown to demethylate histone H3 trimethylated at lysine 9. JMJD2B / KDM4B is physically associated with the MLL2 methyltransferase complex where it serves as a co-factor for ERα-mediated transcriptional activation.

for JMJD2B / KDM4B antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for JMJD2B / KDM4B antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with JMJD2B / KDM4B antibody (pAb).

for JMJD2B / KDM4B antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for JMJD2B / KDM4B antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

JMJD2B / KDM4B antibody (pAb)
 

Data Thumbnails

JMJD2B / KDM4B antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of JMJD2B / KDM4B pAb.
(Click image to enlarge and see details.)

JMJD2B / KDM4B antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of JMJD2B / KDM4B pAb.
(Click image to enlarge and see details.)

JMJD2B / KDM4B antibody (pAb) tested by Western blot.

Western blot of JMJD2B / KDM4B pAb.
(Click image to enlarge and see details.)