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5-Formylcytosine (5-fC) antibody (pAb)

商品コード: 61227 Format: 100 µg ¥67,000 Buy Now
商品コード: 61228 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Reactivity:Other (Not Species Specific)

Applications

Western Blot Validated Dot Blot Validated

for 5-Formylcytosine (5-fC) antibody (pAb)Application Notes


Validated Applications:
DB: 0.5 - 2 µg/ml dilution

Published Applications:
WB
See references for more information. Individual optimization may be required.

for 5-Formylcytosine (5-fC) antibody (pAb)Immunogen

This 5-Formylcytosine antibody was raised against 5-formylcytidine conjugated to KLH and recognizes 5-formylcytosine.

for 5-Formylcytosine (5-fC) antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for 5-Formylcytosine (5-fC) antibody (pAb)References

 
5-Formylcytosine (5-fC) antibody (pAb) tested by dot blot analysis.

Dot blot of 5-Formylcytosine pAb.
Dot blot analysis was used to confirm the specificity of 5-Formylcytosine antibody for 5-Formylcytosine (5-formylcytidine, 5-fC). Single-stranded 38 nt DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) corresponding to the immunogen and related sequences were spotted onto nitrocellulose and probed with the antibody at 0.2 µg/ml. Lane 1: oligo containing 5-carboxylcytidine. Lane 2: oligo containing 5-formylcytidine. Lane 3: oligo containing 5-hydroxymethylcytidine. Lane 4: oligo containing 5-methylcytidine. Lane 5: oligo containing unmodified cytidine.

for 5-Formylcytosine (5-fC) antibody (pAb)Background

DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates.

A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.

for 5-Formylcytosine (5-fC) antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for 5-Formylcytosine (5-fC) antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

5-Formylcytosine (5-fC) antibody (pAb)
 

Data Thumbnails

5-Formylcytosine (5-fC) antibody (pAb) tested by dot blot analysis.

Dot blot of 5-Formylcytosine (5-fC) pAb.
(Click image to enlarge and see details.)