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Antibodies

EKLF antibody (mAb)

Clone: 7B2
Catalog No: 61233 Format: 100 µg $340 Buy Now
Catalog No: 61234 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG3
Purification:Protein G Chromatography
Applications:ChIP, IP, WB
Reactivity:Mouse
Molecular Weight:42 kDa

Applications Table

Western Blot Chromatin IP Immunoprecipitation
Published.
See References below.		 Published.
See References below.		 Published.
See References below.

for EKLF antibody (mAb) (Clone 7B2)Background

The EKLF (Erythroid krueppel like transcription factor, Erythroid transcription factor, KLF1) gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes.

for EKLF antibody (mAb) (Clone 7B2)Immunogen

This EKLF antibody was raised against a full-length recombinant protein to mouse EKLF (Kruppel-like factor 1 (erythroid)).

for EKLF antibody (mAb) (Clone 7B2)Application Notes

This EKLF antibody has been validated for use in chromatin immunoprecipitation, immunoprecipitation and Western blotting. See References for more details.

for EKLF antibody (mAb) (Clone 7B2)Buffer

Purified IgG in 12 mM Tris, pH 8.0, 60 mM Na Citrate, 120 mM NaCl with 30% glycerol and 0.035% sodium azide.

Chromatin IP Assays

Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with EKLF antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use EKLF antibody (mAb).

for EKLF antibody (mAb) (Clone 7B2)References

This antibody has been used as described below:

ChIP and WB: Siatecka M. et al (2010) Mol Cell Biol 30(11):2811-2822
IP: Sengupta T. et al (2009) PNAS 106(11):4213-4218

for EKLF antibody (mAb) (Clone 7B2)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for EKLF antibody (mAb) (Clone 7B2)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting