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Uhrf1 antibody (mAb)

Clone: 1RC-1C10
Aliases: Np95, hNP95, ICBP90, RNF106, hUHRF1, huNp95
商品コード: 61341 Format: 100 µg ¥67,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1k
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:96 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

for Uhrf1 antibody (mAb) (Clone 1RC-1C10)Application Notes


Validated Applications:
ICC/IF: 0.5 µg/ml dilution

Published Applications:
IP
ICC/IF
IHC
WB
See references for more information. Individual optimization may be required.

for Uhrf1 antibody (mAb) (Clone 1RC-1C10)Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acid residue(s) 263-793 of human Uhrf1.

for Uhrf1 antibody (mAb) (Clone 1RC-1C10)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for Uhrf1 antibody (mAb) (Clone 1RC-1C10)References

 
Uhrf1 antibody (mAb) tested by immunofluorescence.

Uhrf1 antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with Uhrf1 antibody at a 0.5 µg/ml dilution.

for Uhrf1 antibody (mAb) (Clone 1RC-1C10)Background

Uhrf1 (ubiquitin-like, containing PHD and RING finger domains 1) is a transcription and cell cycle regulator. It functions as a putative E3 ubiquitin-protein ligase. Uhrf1 is important for G1/S transition of the cell cycle. It is also believed that Uhrf1 may help recruit the DNA methyltransferase, DNMT1, to hemimethylated DNA and assist in the stabilization of DNMT1 with chromatin.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Uhrf1 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Uhrf1 antibody (mAb).

for Uhrf1 antibody (mAb) (Clone 1RC-1C10)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Uhrf1 antibody (mAb) (Clone 1RC-1C10)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Uhrf1 antibody (mAb)
 

Data Thumbnails

Uhrf1 antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of Uhrf1 mAb.
(Click image to enlarge and see details.)