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RNA Pol II CTD phospho Thr4 antibody (mAb)

Clone: 6D7
Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
商品コード: 61361 Format: 100 µg ¥67,000 Buy Now
商品コード: 61362 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2b
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:240 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

for RNA Pol II CTD phospho Thr4 antibody (mAb) (Clone 6D7)Application Notes


Published Applications:
ChIP
IP
ICC/IF
WB
ELISA
See references for more information. Individual optimization may be required.

for RNA Pol II CTD phospho Thr4 antibody (mAb) (Clone 6D7)Immunogen

This antibody was raised against a synthetic peptide corresponding to RNA Pol II CTD phosphorylated at threonine 4.

for RNA Pol II CTD phospho Thr4 antibody (mAb) (Clone 6D7)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for RNA Pol II CTD phospho Thr4 antibody (mAb) (Clone 6D7)References

 
RNA pol II CTD phospho Thr4 antibody (mAb) tested by Western blot.

Western blot of RNA pol II CTD phospho Thr4 mAb.
30 µg of HeLa cell nuclear extract was probed with RNA pol II CTD phospho Thr4 antibody at 2 µg/ml in buffer containing 0.05% Tween.

Specificity testing of RNA pol II CTD phospho Thr4 antibody by bead-based assay.

RNA pol II CTD phospho Thr4 antibody tested by bead-based assay
Luminex bead-based specificity analysis was used to confirm the specificity of RNA pol II CTD phospho Thr4 antibody for RNA pol II CTD peptides conjugated to MagPlex Luminex beads and incubated with 50ng of RNA pol II CTD phospho Thr4 antibody. Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. (Luminex® is a registered trademark of Luminex Corporation.)

for RNA Pol II CTD phospho Thr4 antibody (mAb) (Clone 6D7)Background

RNA pol II (RNA polymerase II) is a complex of 12 proteins that participates in transcription, mRNA processing, and transcription-coupled DNA repair. RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Polo-like kinase 3 (plk3) has been reported to phosphorylate Thr4 which may be required for transcriptional elongation.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with RNA Pol II CTD phospho Thr4 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with RNA Pol II CTD phospho Thr4 antibody (mAb).

for RNA Pol II CTD phospho Thr4 antibody (mAb) (Clone 6D7)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for RNA Pol II CTD phospho Thr4 antibody (mAb) (Clone 6D7)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

RNA Pol II CTD phospho Thr4 antibody (mAb)
 

Data Thumbnails

RNA pol II CTD phospho Thr4 antibody (mAb) tested by Western blot.

Western blot of RNA pol II CTD phospho Thr4 mAb.
(Click image to enlarge and see details.)

Specificity testing of RNA pol II CTD phospho Thr4 antibody by bead-based assay.

Specificity testing of RNA pol II CTD phospho Thr4 antibody by bead-based assay.
Click to enlarge and view details).