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RNA Pol II CTD phospho Tyr1 antibody (mAb)

Clone: 3D12
Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
商品コード: 61383 Format: 100 µg ¥67,000 Buy Now
商品コード: 61384 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:210 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated

for RNA Pol II CTD phospho Tyr1 antibody (mAb) (Clone 3D12)Application Notes


Published Applications:
ChIP
IP
WB
See references for more information. Individual optimization may be required.

for RNA Pol II CTD phospho Tyr1 antibody (mAb) (Clone 3D12)Immunogen

This antibody was raised against a synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.

for RNA Pol II CTD phospho Tyr1 antibody (mAb) (Clone 3D12)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for RNA Pol II CTD phospho Tyr1 antibody (mAb) (Clone 3D12)References

for RNA Pol II CTD phospho Tyr1 antibody (mAb) (Clone 3D12)Background

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at sites within the heptapeptide repeat, at serine 2, serine 5, serine 7 and tyrosine 1. RNA pol II Tyrosine 1 phosphorylation is found during elongation and appears to activate transcription by suppressing RNA pol II termination.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with RNA Pol II CTD phospho Tyr1 antibody (mAb).

for RNA Pol II CTD phospho Tyr1 antibody (mAb) (Clone 3D12)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for RNA Pol II CTD phospho Tyr1 antibody (mAb) (Clone 3D12)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

RNA Pol II CTD phospho Tyr1 antibody (mAb)