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SRF antibody (mAb)

Clone: 2C5
商品コード: 61385 Format: 100 µg ¥69,000 Buy Now
商品コード: 61386 Format: 10 µg ¥20,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:67 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunofluorescence Validated Immunohistochemistry Validated

for SRF antibody (mAb) (Clone 2C5)Application Notes


Validated Applications:
ChIP: 5 - 10 µl per ChIP
ChIP-Seq: 5 - 10 µl each

ChIP-Seq validation was performed by Active Motif's Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here.

Published Applications:
IHC
IF
WB
See references for more information. Individual optimization may be required.

for SRF antibody (mAb) (Clone 2C5)Immunogen

This antibody was raised against a full-length protein corresponding to mouse SRF.

for SRF antibody (mAb) (Clone 2C5)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for SRF antibody (mAb) (Clone 2C5)References

 
SRF antibody (mAb) tested by ChIP-Seq.

SRF antibody (mAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from mouse heart and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify SRF binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

for SRF antibody (mAb) (Clone 2C5)Background

SRF (serum response factor) is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with SRF antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with SRF antibody (mAb).

for SRF antibody (mAb) (Clone 2C5)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SRF antibody (mAb) (Clone 2C5)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SRF antibody (mAb)
 

Data Thumbnails

SRF antibody (mAb) tested by ChIP-Seq.

ChIP-Seq of SRF mAb.
(Click image to enlarge and see details.)