Histone H4K8K12K16 (pan-biotinylated) antibody (pAb)

RRID: AB_2793617
Catalog No: 61393 Format: 100 µl ¥86,000 Buy
Catalog No: 61394 Format: 10 µl ¥24,000 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
None
Host:
Rabbit
Molecular Weight:
8 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Dot Blot Validated

Application Notes

Applications Validated by Active Motif:
DB: 1:500 - 1:2,000 dilution

Immunogen

This antibody was raised against a synthetic peptide containing biotin-Lys 12 of human Histone H4.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

View our Guide to Histone Modifications and Biological Function.

 

Histone H4K8K12K16 (pan-biotinylated) pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4K8K12K16 (pan-biotinylated) antibody. Modified and unmodified peptide were spotted onto the membrane and probed with the antibody.
Lane 1: Peptide biotinylated at lysine 8 of Histone H4.
Lane 2: Peptide biotinylated at lysine 12 of Histone H4.
Lane 3: Peptide biotinylated at lysine 16 of Histone H4.
Lane 4: Unmodified peptide corresponding to amino acids 1-20 of Histone H4.

Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression.

The biotinylation of Histone H4 on Lys8, Lys12 and Lys16 has been reported to be enriched in repeat regions and may participate in repression of transcriptionally competent genes.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting