Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
抗体

Tet3 antibody (pAb)

商品コード: 61395 Format: 100 µl ¥67,000 Buy Now
商品コード: 61396 Format: 10 µl ¥19,000 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:200 kDa
Reactivity:Mouse

Applications

ChIP Validated Western Blot Validated

for Tet3 antibody (pAb)Application Notes


Validated Applications:
WB: 1:1,000 - 1:5,000 dilution

Published Applications:
ChIP
WB

for Tet3 antibody (pAb)Immunogen

This antibody was raised against a recombinant protein corresponding to amino acids 1159-1329 of mouse Tet3.

for Tet3 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for Tet3 antibody (pAb)References

 
Tet3 antibody (pAb) tested by Western blot.

Tet3 antibody (pAb) tested by Western blot.
The blot was probed with Tet3 antibody at a dilution of 1:5,000.

Lane 1: 293T cells transfected with Tet3.
Lane 2: Mouse lung.
Lane 3: Mouse muscle.
Lane 4: Mouse ovary.
Lane 5: Mouse spleen.
Lane 6: Mouse testes.
Lane 7: Mouse thymus.
Lane 8: 293T cells transfected with Tet1.
Lane 9: 293T cells transfected with Tet2.

for Tet3 antibody (pAb)Background

Tet3 (tet methylcytosine dioxygenase 3) catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Conversion into 5hmC initiates a process leading to cytosine demethylation through deamination into 5-hydroxymethyluracil (5hmU) and subsequent replacement by unmethylated cytosine by the base excision repair system. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Tet3 antibody (pAb).

for Tet3 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Tet3 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Tet3 antibody (pAb)
 

Data Thumbnails

Tet3 antibody (pAb) tested by Western blot.

Western blot of Tet3 pAb.
(Click image to enlarge and see details.)