Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
抗体

c-Fos antibody (pAb)

Aliases: p55, AP-1, FOS
商品コード: 61421 Format: 100 µl ¥67,000 Buy Now
商品コード: 61422 Format: 10 µl ¥19,000 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:60 kDa
Reactivity:Human

Applications

Western Blot Validated

for c-Fos antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

for c-Fos antibody (pAb)Immunogen

This antibody was raised against a peptide within human c-Fos.

for c-Fos antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for c-Fos antibody (pAb)References

 
c-Fos antibody (pAb) tested by Western blot.

c-Fos (pAb) tested by Western blot.
c-Fos detection by Western blot. The analysis was performed using 20 µg HeLa nuclear extract (TPA stimulated) and c-Fos at a 1:1,000 dilution.

for c-Fos antibody (pAb)Background

c-Fos (FBJ murine osteosarcoma viral oncogene homolog) is a nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.

for c-Fos antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (TPA stimulated, 2 hr) (Catalog No. 36009) can be used as a positive control for c-Fos antibody.

for c-Fos antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for c-Fos antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

c-Fos antibody (pAb)
 

Data Thumbnails

c-Fos antibody (pAb) tested by Western blot.

Western blot of c-Fos pAb.
(Click image to enlarge and see details.)