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PKM2 antibody (pAb)

Aliases: PK3, TCB, OIP3, PKM, CTHBP, THBP1, HEL-S-30
商品コード: 61423 Format: 100 µl ¥67,000 Buy Now
商品コード: 61424 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:60 kDa
Reactivity:Human

Applications

Western Blot Validated

for PKM2 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for PKM2 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human PKM2.

for PKM2 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol, 0.035% sodium azide and 0.2 mg/ml BSA.

for PKM2 antibody (pAb)References

 
PKM2 antibody (pAb) tested by Western blot.

PKM2 pAb tested by Western blot.
PKM2 detection by Western blot. The analysis was performed using 30 µg HeLa whole cell extract and PKM2 at a 1:1,000 dilution.

for PKM2 antibody (pAb)Background

PKM2 (pyruvate kinase M2 isoform) is a glycolytic enzyme that catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues. The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development, is upregulated in human cancer cells and has been implicated in transcriptional regulation. Recently PKM2 has been described to bind to Histone H3 and phosphorylate threonine 11 (H3T11ph) which implicates PKM2 as an epigenetic regulator of gene expression.

for PKM2 antibody (pAb)Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for PKM2 antibody.

for PKM2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PKM2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PKM2 antibody (pAb)
 

Data Thumbnails

PKM2 antibody (pAb) tested by Western blot.

Western blot of PKM2 pAb.
(Click image to enlarge and see details.)