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TDG / Thymine-DNA glycosylase antibody (pAb)

Aliases: Jza1, JZA-3, E130317C12Rik
商品コード: 61437 Format: 100 µl ¥67,000 Buy Now
商品コード: 61438 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:55 kDa
Reactivity:Mouse

Applications

Western Blot Validated

for TDG / Thymine-DNA glycosylase antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,500 dilution

for TDG / Thymine-DNA glycosylase antibody (pAb)Immunogen

This antibody was raised against a full-length recombinant protein corresponding to mouse thymine DNA glycosylase (TDG).

for TDG / Thymine-DNA glycosylase antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for TDG / Thymine-DNA glycosylase antibody (pAb)References

 
TDG / Thymine-DNA glycosylase antibody (pAb) tested by Western blot.

TDG / Thymine-DNA glycosylase antibody (pAb) tested by Western blot
Nuclear extract of P19 cells (30 µg) probed with TDG / Thymine-DNA glycosylase antibody (1:500).

for TDG / Thymine-DNA glycosylase antibody (pAb)Background

In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. In addition, TET proteins oxidize 5- methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5- formylcytosine (5fC), and 5-carboxylcytosine (5caC). TDG / Thymine-DNA glycosylase functions to correct G/T mispairs to G/C pairs and plays a role in active DNA demethylation by recognizing 5fC and 5caC and converting these to cystosine through the base excision repair (BER) pathway.

for TDG / Thymine-DNA glycosylase antibody (pAb)Positive Control

Active Motif's P19 nuclear extract (Catalog No. 36074) can be used as a positive control for TDG / Thymine-DNA glycosylase antibody.

for TDG / Thymine-DNA glycosylase antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for TDG / Thymine-DNA glycosylase antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

TDG / Thymine-DNA glycosylase antibody (pAb)
 

Data Thumbnails

TDG / Thymine-DNA glycosylase antibody (pAb) tested by Western blot.

Western blot of TDG / Thymine-DNA glycosylase pAb.
(Click image to enlarge and see details.)