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O-GlcNAc antibody (mAb)

Clone: CTD110.6
商品コード: 61453 Format: 100 µg ¥67,000 Buy Now
商品コード: 61454 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgM
Purification:IgM purification
Host:Mouse
Reactivity:Other (Not Species Specific)

Applications

Western Blot Validated Immunoprecipitation Validated

for O-GlcNAc antibody (mAb) (Clone CTD110.6)Application Notes


Published Applications:
IP
WB
See references for more information. Individual optimization may be required.

for O-GlcNAc antibody (mAb) (Clone CTD110.6)Immunogen

This antibody was raised against a peptide containing O-GlcNAc - serine. This antibody recognizes single O-GlcNAc residues in b-O-glycosidic linkage to serine and threonine.

for O-GlcNAc antibody (mAb) (Clone CTD110.6)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for O-GlcNAc antibody (mAb) (Clone CTD110.6)References

for O-GlcNAc antibody (mAb) (Clone CTD110.6)Background

O-GlcNAc (b-O-linked N-acetylglucosamine) is a ubiquitous and dynamic post-translational modification present on numerous cytoplasmic and nuclear proteins and is thought to play a regulatory role in numerous cellular processes including homeostatic mechanisms and gene expression. OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residues whereas OGA / O-GlcNAcase (O-linked N-acetylglucosamine (GlcNAc)-ase) is a glycosidase that removes O-GlcNAc. O-GlcNAc modifications have been described to directly regulate the activities of a variety of transcription factors including Sp1, estrogen receptors, STAT5, NF-κB, p53, YY1, Elf-1, c-Myc, Rb, PDX-1, CREB, fork-head, and others. In addition, RNA polymerase II and the basal transcription factors are also extensively modified by O-GlcNAc. O-GlcNAc modification has been reported to suppress or enhance transcription, depending on the promoter and other associated proteins.

for O-GlcNAc antibody (mAb) (Clone CTD110.6)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for O-GlcNAc antibody (mAb) (Clone CTD110.6)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

O-GlcNAc antibody (mAb)