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DNMT3A antibody (mAb)

Clone: 6A4
Aliases: DNA Methyltransferase 3a, DNMT3A2
商品コード: 61477 Format: 100 µg ¥67,000 Buy Now
商品コード: 61478 Format: 10 µg ¥19,000 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:130 kDa
Reactivity:Human, Mouse, Other (Wide Range)

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for DNMT3A antibody (mAb) (Clone 6A4)Application Notes


Published Applications:
ICC/IF
WB

See references for more information. Individual optimization may be required.

for DNMT3A antibody (mAb) (Clone 6A4)Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acid residues 200 - 215 of human DNMT3A.

for DNMT3A antibody (mAb) (Clone 6A4)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for DNMT3A antibody (mAb) (Clone 6A4)References

for DNMT3A antibody (mAb) (Clone 6A4)Background

DNMT3A (DNA (cytosine-5)-methyltransferase 3A) is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity (By similarity).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with DNMT3A antibody (mAb).

for DNMT3A antibody (mAb) (Clone 6A4)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for DNMT3A antibody (mAb) (Clone 6A4)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

DNMT3A antibody (mAb)