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beta-Catenin antibody (pAb)

Aliases: CTNNB, MRD19, armadillo
商品コード: 61487 Format: 100 µl ¥67,000 Buy Now
商品コード: 61488 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:90 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated

for beta-Catenin antibody (pAb)Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

for beta-Catenin antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of human beta-Catenin.

for beta-Catenin antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for beta-Catenin antibody (pAb)References

 
beta-Catenin antibody (pAb) tested by immunoprecipitation.

beta-Catenin antibody (pAb) tested by Immunoprecipitation.
10 µl of beta-Catenin antibody was used to immunoprecipitate beta-Catenin from 500 µg of HeLa whole cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the beta-Catenin antibody at a dilution of 1:500.

beta-Catenin antibody (pAb) tested by Western blot.

beta-Catenin antibody (pAb) tested by Western blot.
Detection of beta-Catenin antibody by Western blot analysis.
Lane 1: A549 nuclear extract (30 µg).
Lane 2: DU145 nuclear extract (20 µg).
Both probed with beta-Catenin antibody at a dilution of 1:500.

for beta-Catenin antibody (pAb)Background

beta-Catenin is a key downstream component of the canonical Wnt signaling pathway, plays an important role in the regulation of cell adhesion and embryonic development. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of beta-Catenin via BTRC and subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activation of Wnt responsive genes.

for beta-Catenin antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for beta-Catenin antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

beta-Catenin antibody (pAb)
 

Data Thumbnails

beta-Catenin antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of beta-Catenin pAb.
(Click image to enlarge and see details.)

beta-Catenin antibody (pAb) tested by Western blot.

Western blot of beta-Catenin pAb.
(Click image to enlarge and see details.)