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PRMT2 antibody (pAb)

Aliases: HRMT1L1
商品コード: 61491 Format: 100 µl ¥67,000 Buy Now
商品コード: 61492 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:55 kDa
Reactivity:Human

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for PRMT2 antibody (pAb)Application Notes


Validated Applications:
ICC/IF: 1:2,000 dilution
WB: 1:500 - 1:2,500 dilution

for PRMT2 antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of human PRMT2.

for PRMT2 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for PRMT2 antibody (pAb)References

 
PRMT2 antibody (pAb) tested by immunofluorescence.

PRMT2 antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with PRMT2 antibody at a 1:2,000 dilution.

PRMT2 antibody (pAb) tested by Western blot.

PRMT2 antibody (pAb) tested by Western blot.
Nuclear extract of U373 MG cells (20 µg) probed with PRMT2 antibody (pAb) at a dilution of 1:1,000.

for PRMT2 antibody (pAb)Background

PRMT2 (Protein Arginine Methyltransferase 2) is a member of the protein arginine N-methyltransferase (PRMT) family that is capable of monomethylating and asymmetrically dimethylating arginine residues. PRMT2 is an arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with PRMT2 antibody (pAb).

for PRMT2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PRMT2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PRMT2 antibody (pAb)
 

Data Thumbnails

PRMT2 antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of PRMT2 pAb.
(Click image to enlarge and see details.)

PRMT2 antibody (pAb) tested by Western blot.

Western blot of PRMT2 pAb.
(Click image to enlarge and see details.)