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DNMT1 monomethyl Lys142 antibody (pAb)

Aliases: AIM, DNMT, MCMT, CXXC9, HSN1E, ADCADN
商品コード: 61497 Format: 100 µl ¥67,000 Buy Now
商品コード: 61498 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:200 kDa
Reactivity:Human

Applications

Western Blot Validated

for DNMT1 monomethyl Lys142 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Published Applications:

WB

for DNMT1 monomethyl Lys142 antibody (pAb)Immunogen

This antibody was raised against a peptide including monomethyl lysine 142 of human DNMT1.

for DNMT1 monomethyl Lys142 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for DNMT1 monomethyl Lys142 antibody (pAb)References

 
DNMT1 monomethyl Lys142 antibody (pAb) tested by Western blot.

DNMT1 monomethyl Lys142 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (40 µg) probed with DNMT1 monomethyl Lys142 antibody (1:500).

DNMT1 monomethyl Lys142 antibody (pAb) tested by dot blot analysis.

DNMT1 monomethyl Lys142 antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of DNMT1 monomethyl Lys142. Peptides corresponding to the immunogen and the unmodified version of the immunogen were spotted onto PVDF and probed with the antibody at 1:100,000. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: Monomethyl Lys142 peptide.
    Lane 2: Dimethyl lysine 142 peptide.
    Lane 3: Trimethyl lysine142 peptide.
    Lane 4: Unmodified lysine 142 peptide.

for DNMT1 monomethyl Lys142 antibody (pAb)Background

DNMT1 (DNA (Cytosine-5-)-Methyltransferase 1) methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Methylation at lysine 142 by SETD7 promotes DNMT1 proteasomal degradation while phosphorylation of serine 143 via AKT blocks degradation. These modifications appear to play an important role in regulating DNMT enzyme activity and thus levels of DNA methylation.

for DNMT1 monomethyl Lys142 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for DNMT1 monomethyl Lys142 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

DNMT1 monomethyl Lys142 antibody (pAb)
 

Data Thumbnails

DNMT1 monomethyl Lys142 antibody (pAb) tested by Western blot.

Western blot of DNMT1 monomethyl Lys142 pAb.
(Click image to enlarge and see details.)

DNMT1 monomethyl Lys142 antibody (pAb) tested by dot blot analysis.

Dot blot of DNMT1 monomethyl Lys142 pAb.
(Click image to enlarge and see details.)