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ATF1 antibody (pAb)

Aliases: TREB36, EWS-ATF1, FUS/ATF-1
商品コード: 61563 Format: 100 µl ¥67,000 Buy Now
商品コード: 61564 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:40 kDa
Reactivity:Human

Applications

Western Blot Validated

for ATF1 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for ATF1 antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human ATF1.

for ATF1 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for ATF1 antibody (pAb)References

 
ATF1 antibody (pAb) tested by Western blot.

ATF1 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (30 µg) probed with ATF1 antibody (pAb) at a dilution of 1:500.

for ATF1 antibody (pAb)Background

ATF1 (Activating Transcription Factor 1) binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.

for ATF1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for ATF1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

ATF1 antibody (pAb)
 

Data Thumbnails

ATF1 antibody (pAb) tested by Western blot.

Western blot of ATF1 pAb.
(Click image to enlarge and see details.)