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REST antibody (pAb)

商品コード: 61571 Format: 100 µl ¥67,000 Buy Now
商品コード: 61572 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:160 kDa
Reactivity:Human

Applications

Western Blot Validated

for REST antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for REST antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human REST.

for REST antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for REST antibody (pAb)References

 
REST antibody (pAb) tested by Western blot.

REST antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (40 µg) probed with REST antibody (1:500).

for REST antibody (pAb)Background

REST (RE1-silencing transcription factor) is a transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.

for REST antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for REST antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

REST antibody (pAb)
 

Data Thumbnails

REST antibody (pAb) tested by Western blot.

Western blot of REST pAb.
(Click image to enlarge and see details.)