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TCF7 antibody (pAb)

Aliases: TCF-1
商品コード: 61579 Format: 100 µl ¥67,000 Buy Now
商品コード: 61580 Format: 10 µl ¥19,000 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:52 & 55 kDa
Reactivity:Human

Applications

Western Blot Validated

for TCF7 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for TCF7 antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of human TCF7.

for TCF7 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for TCF7 antibody (pAb)References

 
TCF7 antibody (pAb) tested by Western blot.

TCF7 antibody (pAb) tested by Western blot.
TCF7 antibody detection by Western blot. The analysis was performed using 25 µg Molt-4 nuclear extract and TCF7 antibody at a 1:500 dilution.

for TCF7 antibody (pAb)Background

TCF7 (Transcription Factor 7) is a transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.

for TCF7 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for TCF7 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

TCF7 antibody (pAb)
 

Data Thumbnails

TCF7 antibody (pAb) tested by Western blot.

Western blot of TCF7 pAb.
(Click image to enlarge and see details.)